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Original Article

Comparative electrophoresis, isoelectric focusing and numerical taxonomy of some isolates of Fonsecaea pedrosoi and allied fungi

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Pages 253-264 | Accepted 22 Mar 1985, Published online: 09 Jul 2009
 

Abstract

The proteins in broken-cell extracts from eight isolates of Fonsecaea pedrosoi, the principal agent of chromomycosis, were studied and compared by electrophoresis and isoelectric focusing. A type pattern was established with 16 fractions ranging in molecular weight between 7600 and 78500 daltons and 16 fractions varying in isoelectric point between 4·95 and 7·90. A genetic distance of 0·1 found by the numerical study applied to both analyses reveals a considerable similarity among the isolates studied. This resemblance was moreover observed between F. pedrosoi and other related dematiaceous fungi.

RESUME

Les protéines de 8 souches de Fonsecaea pedrosoi, agent principal de chromomycose, ont été étudiées et comparées par électrophorèse en SDS et isoélectrofocalisation. Un profil type a été établi avec 16 fractions dont les poids moléculaires sont compris entre 7600 et 78500 daltons et 16 fractions dont les points isoélectriques varient entre 4·95 et 7·90.

Une distance génétique voisine de 0,1 obtenue par analyse numérique révèle une similarité considérable entre ces souches. De plus cette ressemblance a été observée entre F. pedrosoi et d'autres champignons dématiées apparentés.

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