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Original

Comparison of different protocols for 13C‐urea breath test for the diagnosis of Helicobacter pylori infection in healthy volunteers

, , , , , , & show all
Pages 491-498 | Received 28 Dec 2004, Accepted 24 May 2005, Published online: 08 Jul 2009
 

Abstract

Objective. The 13C‐urea breath test (13C‐UBT) is the most accurate non‐invasive method for diagnosis of Helicobacter pylori infection. However, several methodological issues have not been resolved yet. The aim of this study was to test different protocols of 13C‐UBT to find the optimal test drink and sampling interval. Material and methods. 13C‐UBT was performed at 3‐day intervals in 27 healthy volunteers using citric acid (test A), orange juice (B) and still water (C) as test drinks. Breath samples were collected from time 5 to 60 min. A total number of 2106 breath samples were analysed by isotope ratio mass spectrometry (cut‐off value 3.5). Results. Differences in delta values were greater than would be expected by chance (A versus B and A versus C at times 20, 25, 30, 35 and 40 min, p<0.05, Dunnett's method). There were no grey zone‐ or false‐negative results among H. pylori‐positive persons in test A at any time, but some were found in tests B and C. Optimal intervals for breath sampling are at times 20 or 25 min after 13C‐urea ingestion. Conclusions. Citric acid solution as a test drink and 20‐ or 25‐min breath sampling intervals are optimal for the 13C‐UBT in healthy volunteers.

Acknowledgement

This study was supported by a grant from the Ministry of Health of the Czech Republic MZ VZ 000179906.

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