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ORIGINAL ARTICLE

Detection of MTRR 66A→G polymorphism using the real‐time polymerase chain reaction machine LightCycler for determination of composition of allele after restriction cleavage

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Pages 685-694 | Received 19 Sep 2005, Accepted 12 Jul 2006, Published online: 08 Jul 2009
 

Abstract

The MTRR gene codes for methionine synthase reductase, one of the enzymes involved in the conversion of homocysteine to methionine. This conversion influences the overall level of total plasma homocysteine (tHcy) and mutations, which reduces the enzyme activity and results in an increased concentration of tHcy. A high homocysteine level is a well‐documented independent risk factor for cardiovascular disease. A polymorphism in the gene for methionine synthase reductase (MTRR 66 A>G) has been shown to be associated with the risk of giving birth to a child with Down's syndrome, and the risk of having a foetus with neural tube defects. We have established a method for analysing MTRR 66A>G on DNA from dried blood spots using melting temperature analysis. The DNA was extracted from dried blood spots using a fast procedure by boiling only.

Acknowledgements

We thank Per Jørgensen Odense University Hospital for invaluable help and his supervision of K. C. Tvedegaard. We also thank Mette Gaustadnes for advice and assistance.

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