A new approach for diagnosing chronic myelomonocytic leukemia using structural parameters of Sysmex XN^TM analyzers in routine laboratory practice

Abstract

According to WHO recommendations, diagnosis of chronic myelomonocytic leukemia (CMML) beforehand requires microscopic examination of peripheral blood to identify dysplasia and/or blasts when monocytes are greater or equal to 1.0 × 10⁹/L and 10% of leucocytes. We analyzed parameters derived from Sysmex^TM XN analyzers to improve the management of microscopic examination for monocytosis. We analyzed results of the complete blood count and the positioning and dispersion parameters of polymorphonuclear neutrophils and monocytes in 61 patients presenting with CMML and 635 control patients presenting with a reactive monocytosis. We used logistic regression and multivariate analysis to define a score for smear review. Three parameters were selected: neutrophil/monocyte ratio, structural neutrophil dispersion (Ne-WX) and monocyte absolute value. We established an equation in which the threshold of 0.160 guided microscopic examination in the search for CMML abnormalities with a sensitivity of 0.967 and a specificity of 0.978 in the learning cohort (696 samples) and 0.923 and 0.936 in the validation cohort (1809 samples) respectively. We created a score for microscopic smear examination of patients presenting with a monocytosis greater or equal to 1.0 × 10⁹/L and 10% of leucocytes, improving efficiency in laboratory routine practice.

Keywords:

• Hematology
• chronic myelomonocytic leukemia
• disease diagnostics
• microscopy
• automated blood count

Acknowledgements

We thank the biologists and technicians from the hematology laboratory at the Etablissement Français du Sang who analyzed the Besançon cases. Jean-Pierre Pérol is employed by Sysmex Europe GMBH and provided scientific and technical expertise on XN analyzers. We thank Audrey Seigeot for her preliminary study on XN parameters at Besançon laboratory. We thank Elizabeth Wager for language editing.

Disclosure statement

No potential conflict of interest was reported by the authors.