Homocysteine and related B-vitamin status in coeliac disease: Effects of gluten exclusion and histological recovery

Abstract

Objective. Hyperhomocysteinaemia is considered to be a risk factor for cardiovascular disease (particularly stroke) and has been implicated in recurrent miscarriage and osteoporotic fracture, recognized manifestations of coeliac disease (CD). The objective of this study was to compare plasma homocysteine levels and biomarker status of metabolically related B vitamins (folate, vitamin B₁₂, B₆ and riboflavin) in treated and untreated CD patients and healthy controls. Material and methods. CD patients attending a clinic for either initial or follow-up biopsy (at least 12 months after commencing a gluten-free diet) were categorized into three groups: 1) newly diagnosed (untreated; n=35); 2) persistent villous atrophy (VA) at follow-up (n=24) or 3) recovered VA at follow-up (n=41). Blood samples were analysed for plasma homocysteine, serum and red cell folate and serum vitamin B₁₂ levels, and for plasma pyridoxal 5-phosphate (PLP, vitamin B₆) and riboflavin (vitamin B₂) status. Results. Homocysteine concentrations were significantly higher (p=0.05) and red cell and serum folate significantly lower (p<0.001) in untreated patients compared with controls, while all three reached normal levels in recovered VA patients. Although untreated and persistent VA patients tended to have lower B₁₂ levels, these did not reach significance. There was no evidence of compromised B₆ or riboflavin status, even in untreated CD patients. Homocysteine concentrations were inversely associated with both serum (r=−0.421; p<0.001) and red cell (r=−0.459; p<0.001) folate and with serum vitamin B₁₂ (r=−0.353; p=0.001). Conclusions. Gluten exclusion in CD improves folate status and normalizes homocysteine concentrations. Reducing the risk of homocysteine-related disease may be another reason for aggressive diagnosis and treatment of CD.

• B vitamins
• coeliac disease
• dietetics
• folic acid
• gluten
• homocysteine
• malabsorption
• villous atrophy