Studies on the Reversibility of Oral Trypsin Inhibitor Induced Changes of Rat Pancreatic Exocrine Enzyme Activity and Insulin Secretory Capacity

Abstract

Ihse, I., Arnesjö, B. & Lundquist, I. Studies on the reversibility of oral trypsin inhibitor induced changes of rat pancreatic exocrine enzyme activity and insulin secretory capacity. Scand. J. Gastroent. 1975, 10, 321-326.

Rats were given a daily dose of bovine trypsin inhibitor for 3 weeks (Group 3 A) or 8 weeks (Group 8 A) via an orogastric tube. In another rat group the trypsin inhibitor after 3 weeks was replaced by water for another 5 weeks (Group 8 B). In group 3 A and 8 A an enlargement of the pancreatic gland was found. In group 3 A and 8 A the pancreatic protein was increased. In group 3 A and 8 A the pancreatic trypsinogen increased in a more pronounced way than the amylase, while the pancreatic lipase was found to be uninfluenced by the trypsin inhibitor treatment. Five weeks after the cessation of a 3-week treatment the only remaining effect on the exocrine pancreas was an increase of the pancreatic trypsinogen. In group 3 A an impairment of insulin secretion following intravenous L-isopropylnoradrenaline and gliben-clamide was found. Glucose levels were similar to those of the control rats. Furthermore, a slight decrease of the pancreatic insulin content per g protein was found in these rats. In group 8 A and 8 B, the insulin secretory pattern was found to be normal following intravenous glucose administration, and an increased glucose elimination rate (k-value) compared with the control group suggested a favourable effect of oral trypsin inhibitor treatment on the glucose tolerance.

Key-words::

• Endocrine pancreas
• enzyme activity
• exocrine pancreas
• glucose tolerance
• insulin secretion
• oral trypsin inhibitor
• rats