The lncRNA TRG-AS1 promotes the growth of colorectal cancer cells through the regulation of P2RY10/GNA13

Abstract

Background

The lncRNA TRG-AS1 and its co-expressed gene P2RY10 are important for colorectal cancer (CRC) occurrence and development. The purpose of our research was to explore the roles of TRG-AS1 and P2RY10 in CRC progression.

Methods

The abundance of TRG-AS1 and P2RY10 in CRC cell lines (HT-29 and LoVo) and normal colon cells FHC was determined and difference between CRC cells and normal cells was compared. LoVo cells were transfected with si-TRG-AS1 and si-P2RY10 constructs. Subsequently, the viability, colony formation, and migration of the transfected cells were analyzed using cell counting kit-8, clonogenicity, and scratch-wound/Transwell® assays, respectively. Cells overexpressing GNA13 were used to further explore the relationship between TRG-AS1 and P2RY10 along with their downstream functions. Finally, nude mice were injected with different transfected cell types to observe tumor formation in vivo.

Results

TRG-AS1 and P2RY10 were significantly upregulated in HT-29 and LoVo compared to FHC cells. TRG-AS1 knockdown and P2RY10 silencing suppressed the viability, colony formation, and migration of LoVo cells. TRG-AS1 knockdown downregulated the expression of P2RY10, GNA12, and GNA13, while P2RY10 silencing downregulated the expression of TRG-AS1, GNA12, and GNA13. Additionally, GNA13 overexpression reversed the cell growth and gene expression changes in LoVo cells induced by TRG-AS1 knockdown or P2RY10 silencing. In vivo experiments revealed that CRC tumor growth was suppressed by TRG-AS1 knockdown and P2RY10 silencing.

Conclusions

TRG-AS1 knockdown repressed the growth of HT-29 and LoVo by regulating P2RY10 and GNA13 expression.

Keywords:

• Colorectal cancer
• trg-as1
• LoVo cells
• P2RY10
• GNA13

Acknowledgments

We are thankful to our laboratory colleagues for their assistance in our research. This study was approved by the Ethics Committee of Changzhou First People’s Hospital.

Author contributions

DLS, LZ, LQS and HZ conceived and designed the experiments. LZ, BYL, QZ, YJL and LHD performed the experiments. LQS, BYL, QZ and LHD analyzed the data. LZ and HZ wrote the article.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the Medical Research Project of Jiangsu Health Commission (No. Z2019027), the Young Talent Development Plan of Changzhou Health Commission (CZQM2021028), the Science and Technology Project of Changzhou Health Commission (No. QN202338), Changzhou Applied Basic Research Program (CJ20220231).