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Scandinavian Journal of Infectious Diseases Volume 38, 2006 - Issue 10
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CASE REPORT

DNA sequence-based detection of group B Streptococcus directly from heart valve tissue in a patient with culture-negative endocarditis

Kelly Guggisberg From the Departments of Pathology & Laboratory Medicine, Calgary, Alberta, Canada
,
Robin Parker From the Departments of Pathology & Laboratory Medicine, Calgary, Alberta, Canada; Divisions of Anatomic Pathology, Calgary, Alberta, Canada
,
David Megran Medicine, Calgary, Alberta, Canada; Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
,
Kunyan Zhang From the Departments of Pathology & Laboratory Medicine, Calgary, Alberta, Canada; Medicine, Calgary, Alberta, Canada; Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada; Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada
,
Wanda Lester From the Departments of Pathology & Laboratory Medicine, Calgary, Alberta, Canada; Divisions of Anatomic Pathology, Calgary, Alberta, Canada
&
Sameer Elsayed From the Departments of Pathology & Laboratory Medicine, Calgary, Alberta, Canada; Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada; Microbiology, Calgary Laboratory Services, Calgary, Alberta, CanadaCorrespondence[email protected]
Pages 931-934 | Received 23 Nov 2005, Published online: 08 Jul 2009
 
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Abstract

A human case of culture-negative bacterial endocarditis is presented where conventional methods failed to determine a microbial aetiology. DNA sequencing performed directly on autopsy heart valve tissue revealed Streptococcus agalactiae (group B streptococcus). To our knowledge, this is the first report of DNA sequence-based detection of this organism directly from a heart valve.

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