Aluminum-tolerance of carrot (daucus carota L.) plants regenerated from selected cell cultures

Abstract

Aluminum-tolerant variants from cell cultures of carrot (Daucus carota L. cv. MS Yonsun) were selected by exposing them to excess ionic aluminum. The medium contained A1CI₃ and 0.1 mM phosphate at pH 4.0. Under ionic aluminum stress (0.5-1.0 mM), 282 calli were selected. Twenty-eight fertile plants were regenerated from 7 selected calli. Based on the root elongation tests of the seedlings, the seeds displayed aluminum-tolerance. In the case of the cell line H7-08, the selection occurred at a frequency of ca. 6 × 10^-7 when the materials were plated on agar medium containing 0.5 mM aluminum.

Key Words:

• aluminum-tolerance
• carrot cells
• ionic aluminum