Possible involvement of nitric oxide in promoting the initial growth of rice seedlings at low temperature by inoculation of Bacillus pumilus strain TUAT1 spores

ABSTRACT

Bacillus pumilus strain TUAT1, a type of plant growth – promoting rhizobacteria (PGPR), is used as an ingredient in biofertilizer. Although we confirmed that B. pumilus TUAT1 promotes the growth of rice seedlings at 25°C, this effect has not been shown at lower temperatures. In this study, we confirmed that inoculation of rice seedlings with spores of B. pumilus TUAT1 promoted seedling emergence from soil and subsequent growth at 15°C. Except for the effect on root growth, these effects disappeared when seeds were treated with 2–4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, a nitric oxide (NO) scavenger, simultaneously with B. pumilus TUAT1 inoculation. Increased NO accumulation was detected in seed embryos 3 h after inoculation, suggesting that NO plays a role in the effects of B. pumilus TUAT1 inoculation. Transcriptome analysis using RNA-seq suggested the involvement of immune responses and cytochrome respiratory pathways in increasing NO levels in embryos after inoculation with B. pumilus TUAT1. Transcriptome analysis also indicated that transcription of genes involved in cold tolerance was accelerated in embryos following the increase in NO resulting from inoculation with B. pumilus TUAT1. These findings suggest that a novel NO signaling mechanism is involved in PGPR-induced growth promotion in plants. These results also indicate that inoculation with B. pumilus TUAT1 spores may help overcome the initial growth failure of seedlings in direct sowing culture of rice in cold regions.

KEY WORDS:

• Alternative oxidase (AOX) pathway
• seedling emergence
• low temperature
• nitric oxide
• plant growth–promoting rhizobacteria (PGPR)

Acknowledgments

We acknowledge the support of the Japan Society for the Promotion of Science (JSPS), the Ministry of Agriculture, Forestry and Fisheries (MAFF) of Japan, and the Bio-oriented Technology Research Advancement Institution (BRAIN).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/00380768.2023.2240834

Additional information

Funding

This study was supported by JSPS KAKENHI (the Grant-in-Aid for Challenging Research, Exploratory, JP20K21267), MAFF of Japan (the Research Promotion Program of Science and Technology for Agriculture, Forestry and Fisheries Industry and Food Industry, 26073C), and BRAIN (Special Scheme Project on Regional Developing Strategy, 16822446).