Molecular encapsulation of amodiaquine in 2-hydroxypropyl β-cyclodextrin cavity. Characterization and its in vitro cytotoxicity

ABSTRACT

Amodiaquine has been used widely as an antimalarial drug. Amodiaquine is a mannich base 4-amino quinolone with a mode of action similar to that of chloroquine. The inclusion complex of amodiaquine with 2-(hydroxypropyl)-beta-cyclodextrin in solution phase is studied from the ground and excited state with absorption and fluorescence spectroscopic techniques, respectively. A binding constant and stoichiometric ratio between amodiaquine and 2-(hydroxypropyl)-beta-cyclodextrin are calculated by the use of Benesi–Hildebrand equation. The solid complexes are prepared by physical, kneading and coprecipitation methods. The solid complexes are characterized by Fourier-transform infrared spectral analysis, Differential scanning calorimetric curves and powder X-ray diffraction patterns. The anticancer activity was tested for pure amodiaquine and their complex with 2-(hydroxypropyl)-beta-cyclodextrin against MDA MB 231 cell line. It clearly showed that a significant improvement of anticancer activity of amodiaquine when forming a complex with 2-(hydroxypropyl)-beta-cyclodextrin.

KEYWORDS:

• 2-(Hydroxypropyl)-β-cyclodextrin
• amodiaquine
• characterization
• differential scanning calorimetry
• in vitro cytotoxicity