Abstract
Pyrophosphate-linked coenzymes play essential roles in several biochemical systems. Symmetrical diadenosine-5′-pyrophosphate (Ap2A) has been synthesized from adenosine-5′-phosphate in virtually quantitative yield. The simple procedure is carried out in anhydrous pyridine using adenosine phosphoromorpholidate and adenosine monophosphate bis-(tri-n-butylammonium salt) as coupling reagents.
ACKNOWLEDGMENTS
The authors are grateful for the help and technical assistance of Andrea Armirotti. This work was supported by grants from the Italian Association for Cancer Research (AIRC), the Italian Ministry for Education, University and Scientific Research (Grants MIUR-PRIN 2003, MIUR FIRB RBNE01ERXR, and MIUR FIRB RBLA039LSF), and the Fondazione Cassa di Risparmio di Genova e Imperia.