In-vitro antibacterial activity of Ni(II), Cu(II), and Zn(II) complexes incorporating new azo-azomethine ligand possessing excellent antioxidant, anti-inflammatory activity and protective effect of free radicals against plasmid DNA

Abstract

Azo Schiff base ligand 2-hydroxy-3-methoxy-5-(tolyldiazenyl)benzaldehyde oxime (HL¹) and 2-hydroxy-3-methoxy-5-(methoxyphenyl)benzaldehyde oxime (HL²) were prepared along with their transition metal complexes of Ni(II), Cu(II), and Zn(II). Ligands and their metal complexes were characterized by several analysis techniques. In- vitro antibacterial, antioxidant and anti-inflammatory activities of synthesized ligands and their metal complexes have been studied. Biological study showed that amongst all the synthesized compounds, Cu(II) complexes possessed excellent antibacterial activity than standard antibiotic Chloramphenicol. Ligands (HL¹) and (HL²) showed excellent antioxidant as well as anti-inflammatory activity. Both the ligands were tested for their protective effect of free radicals against plasmid DNA and it was found that both the ligands showed good DNA nicking activity.

Graphical Abstract

Keywords:

• Antibacterial
• anti-inflammatory
• antioxidant
• azo-Schiff base
• DNA fragmentation
• metal complexes

Acknowledgments

One of the authors (Mangesh Kasare) is thankful to DST-PURSE, New Delhi for the award of research fellowship. All authors are also thankful to Microanalytical Laboratory, Department of Chemistry, University of Mumbai for providing characterization facilities and IIT Bombay SAIF for ESR facility.

Disclosure statement

No potential conflict of interest was reported by the authors.