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Abstract
The indirect determination of cysteine in wools by atomic–absorption spectrophotometry (AAS) is studied, and the results are compared with those obtained by other spectrophotometric methods. From the hydrolysate of wools by reduction of Cu(II) by cysteine in the presence of thiocyanate, a precipitate of CuSCN is obtained. This precipitate is dissolved in an acidic medium, after which the copper concentration is determined by ASS.
The method proposed (AAS) offers advantages over those with which it is compared (Folin's and Ellman's) with regard to accuracy and precision and also in that it does not require cysteine standards.