Detection and isolation of Helicobacter mustelae from stoats in New Zealand

Abstract

AIM: To determine the incidence of Helicobacter mustelae in stoats (Mustela erminea) in New Zealand.

METHODS: Helicobacter-like organisms and total genomic DNA were isolated from gastric tissue of stoats and identified using a combination of bacterial culture, phenotypic testing and molecular techniques.

RESULTS: A Helicobacter-specific 16S rRNA polymerase chain reaction product was detected in 16/32 gastric tissue biopsies tested. Nine of 13 partially sequenced 16S rRNA DNA identified H. mustelae 16S DNA. Bacteria, subsequently identified as H. mustelae, were successfully cultured from the stomachs of 4/32 stoats. Other Helicobacter species were also identified by DNA sequence analysis, but were not cultured.

CONCLUSIONS: Helicobacter mustelae is present in stoats from both the North and South Islands of New Zealand.

KEYWORDS:

• Stoats
• Helicobacter mustelae