Use of polymerase chain reaction for the differentiation of Group A bovine rotavirus G6, G8, and G10 genotypes in the North Island of New Zealand

Abstract

AIM: To determine the presence of Group A rotavirus G6, G8, and G10 genotypes in calves in the North Island of New Zealand.

METHODS: Faecal samples from 730 calves (<6 weeks old) with diarrhoea were collected during 2006 and 2007 from seven regions in the North Island of New Zealand. The samples were screened for the presence of Group A rotavirus antigen, using a commercial ELISA. Forty-one samples from different farms were randomly selected out of the 385 ELISA-positive samples and tested using PCR for the presence of G6, G8, and G10 genotypes of rotavirus.

RESULTS: The PCR analysis of 41 antigen-positive field samples revealed that 37 (90%) contained genotype G6, three (7%) genotype G10, one sample (2%) had both G6 and G10 genotypes, and none contained genotype G8.

CONCLUSIONS: Rotavirus genotype G6 was the predominant genotype found in this preliminary study and was present in all seven regions studied. Genotype G10 was also found in some regions of the North Island, whereas genotype G8 was not found in any sample.

CLINICAL RELEVANCE: This is the first report on rotavirus G genotypes present in calves in the North Island of New Zealand.

KEY WORDS:

• Group A rotavirus
• G6
• G10
• calves
• New Zealand
• genotype

Acknowledgements

We wish to thank Gribbles Laboratory and New Zealand Veterinary Pathology for their help collecting samples. We also wish to thank Dr Jianning Wang, Ministry of Agriculture and Forestry, New Zealand, for the cell-cultured rotavirus isolate.