Investigation of a pig herd with animals seropositive for classical swine fever and where porcine circovirus-associated disease had been diagnosed

Abstract

AIM: To investigate the cause of classical swine fever (CSF) virus-seropositive animals in a nucleus pig-breeding herd in New Zealand, where porcine circovirus-associated disease had been diagnosed.

CASE HISTORY AND CLINICAL FINDINGS: An exotic disease investigation was undertaken to exclude CSF and porcine reproductive and respiratory syndrome (PRRS) on a nucleus pig-breeding herd comprising approximately 300 breeding sows, 1,000 weaners, and 650 grower pigs. The herd was experiencing poor reproductive performance in sows, and breeding records showed a declining farrowing rate attributable to a single manager. The growing pigs (10–15 weeks old) were experiencing respiratory disease and wasting, and the mortality rate by pen varied between 9 and 20%. Post-mortem changes in affected grower pigs were consistent with circovirus-associated diseases.

DIAGNOSTIC TESTING: Serological screening using an IDEXX-ELISA gave negative results for PRRS virus antibodies, but two grower pigs and one sow tested positive for CSF virus antibodies. These three seropositive animals remained positive to CSF virus, using three commercial ELISA test kits, over 27 weeks. A newly developed virus neutralisation test (VNT), using a New Zealand isolate of border disease (BD) virus, demonstrated that the seropositive pig sera had higher antibody titres to BD virus than to bovine viral diarrhoea (BVD) virus and CSF virus.

PCR performed on tonsil, kidney, ileum and spleen gave negative results for CSF virus, and histopathology on lymph nodes, intestine, lung, kidney, liver and brain showed no evidence of the disease. Virus isolation performed on a number of samples was negative.

CLINICAL RELEVANCE: The seropositive samples for CSF virus found in this investigation were likely to be a cross reaction to a pestivirus other than CSF virus. The finding of a possible endemic pestivirus capable of being transmitted between sheep and pigs on this farm may explain findings from previous serological survey work in New Zealand, and supports experience elsewhere, where BD virus was found to be the predominant ruminant pestivirus infecting pigs. The results show that pestivirus cross reactivity can result in unexpectedly high titres, and that testing with a full set of (local) pestiviruses is necessary to reach the correct conclusion. The investigation has direct relevance where pig herds with a low seroprevalence are encountered during surveillance for CSF.

KEY WORDS:

• Border disease
• classical swine fever
• disease investigation
• serology
• virus neutralisation test
• VNT
• enzyme-linked immunosorbent assay
• ELISA

Acknowledgements

The authors would like to thank Dr Willie Loeffen, Central Institute for Animal Disease Control, Lelystad, Netherlands, for providing positive sera for BD virus, and for technical advice; Alastair Johnstone, Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand, for providing pathological services; Dr Bruce Welch, Pig Veterinary Services, Christchurch, New Zealand, for on-farm veterinary assistance and technical advice; and all staff at the IDC, Wallaceville, Upper Hutt, New Zealand, for their assistance with laboratory testing. The authors are also grateful to IDEXX Laboratories, the Institute Pourquier and Cedi Diagnostics, for supplying trial kits free-of-charge.

Notes

¹ M Zalunardo, IDEXX Laboratories, Rydalmere, NSW, Australia

² D Gardinaru, Institute Pourquier, Montpellier, France