Preliminary investigation of the effect of treating sheep during pregnancy with a vitamin A, D, E formulation on the incidence of vaginal prolapse

ABSTRACT

Aim: To determine if vitamin D₃ treatment reduced the incidence of vaginal prolapse in pregnant sheep on a North Canterbury sheep breeding property.

Methods: Pregnant ewes from a single farm were allocated to three treatment groups in May 2018. At this time, the first group (EarlyVitADE; n = 512) received an I/M 1 mL dose of 500,000 IU/mL vitamin D₃, 60,000 IU/mL vitamin A, and 25 mg/mL vitamin E. This was repeated in July 2018, when the second group (LateVitADE; n = 695) also received the same treatment. The third group (n = 737) were untreated controls. All cases of vaginal prolapse on the property were recorded from pregnancy diagnosis in June 2018 until ewes were set-stocked in August 2018. The planned start of lambing was 10 August 2018.

Results: During the period of observation, vaginal prolapses were recorded in 3/699 (0.4%) 2-year-old ewes, and the odds of vaginal prolapse were not associated with treatment group in these ewes (p > 0.3). Amongst ewes aged ≥3 years, during the same period, there were 6/333 (1.8%), 6/443 (1.4%) and 25/469 (5.3%) cases in the EarlyVitADE, LateVitADE and control groups, respectively. Compared to control ewes, the odds of vaginal prolapse were reduced in both the EarlyVitADE (OR = 0.37; 95% CI = 0.15–0.92) and LateVitADE (OR = 0.25; 95% CI = 0.10–0.62) treatment groups.

Conclusions and clinical relevance: In this preliminary study, administration of injectable vitamins A, D₃, and E to pregnant ewes reduced the incidence of vaginal prolapse during the period from pregnancy diagnosis to set-stocking on one North Canterbury hill-country farm. Due to the restricted data collection period, this investigation should be replicated to better quantify the repeatability of the observed treatment effect over the complete lambing period.

KEYWORDS:

• Vitamin D
• vaginal prolapse
• sheep
• muscle
• pregnancy

Acknowledgements

The authors would like to thank the North Canterbury farmer, on whose farm this trial was performed, for his significant assistance in the design of the trial, bringing in stock, assisting with administration of product, and in funding the vitamin product used during the trial. The farmer wishes to remain anonymous. North Canterbury Veterinary Clinics supported this trial with significant in-kind contribution of time, materials, and technical expertise. Funding for plasma calcium, phosphorus and vitamin D analyses was obtained from the School of Veterinary Science McGeorge Fund, Massey University.

ORCID

KE Dittmer http://orcid.org/0000-0002-1813-2197