Neosporosis in bulls: potential for venereal transmission, and effect on semen quality and production

ABSTRACT

Neospora caninum caninum is a protozoan parasite that can cause abortions, birth of congenitally infected calves, and reduced milk production in cattle. Neosporosis in cattle can be transmitted through vertical transplacental transmission from dam to calf, or through horizontal transmission between animals. While it is well established that horizontal transmission from dogs (the definitive host) to cattle (an intermediate host) occurs, it is not clear whether horizontal transmission may occur between cows and bulls during mating. Transmission via infected semen of seropositive bulls was suggested when N. caninum DNA was found in bull semen. The primary objective of this article is to critically evaluate the evidence for venereal transmission of N. caninum between cattle, to determine whether this transmission route is likely to occur. A secondary objective is to review and evaluate published evidence for whether neosporosis in bulls may affect semen quality and production. N. caninum DNA can be detected by PCR in occasional batches of semen straws from bulls that are seropositive for N. caninum. Insemination of heifers with semen experimentally infected with 5 × 10⁴ N. caninum tachyzoites has caused persistent seroconversion in some animals. However, the highest concentration of parasites in the semen of naturally infected bulls was estimated, using quantitative PCR, as 10 tachyzoites. Thus it appears that, whether used for natural or artificial insemination, the semen of bulls naturally infected with N. caninum, does not contain the necessary intrauterine dose of tachyzoites to cause persistent seroconversion in a heifer or cow. Thus, based on the available evidence, the venereal transmission of N. caninum via bull semen is extremely unlikely. Nevertheless, neosporosis in bulls may influence semen quality and production. Epididymal semen concentration, viability, and motility appeared “to be” significantly decreased in N. caninum-seropositive bulls compared to seronegative bulls. Furthermore, semen production could be altered if neosporosis affects the thyroid gland. The thyroid hormones such as triiodothyronine and thyroxine (T4) may affect testicular development. Significantly lower concentrations of T4 were observed in the blood of N. caninum-seropositive, compared with those of seronegative bulls. Longitudinal studies are needed to confirm the long-term effect and significance of neosporosis on semen quality and thyroid hormone concentrations in bulls.

KEYWORDS:

• Bulls
• neosporosis
• venereal transmission
• semen quality
• semen production

Acknowledgements

Thank you to my colleagues Dr WB Hancock and Dr J Lorenz as well as Dr S Hendriks and Dr L Sevillano from Massey University for their invaluable comments that helped shape this review. Also, thanks to Cambridge Veterinary Services for funding the postgraduate study that inspired me to write the review.