Development of an intrauterine infection model in the postpartum dairy cow

ABSTRACT

Aims

To develop an intrauterine infection model for Trueperella pyogenes in postpartum dairy cows and to assess the effect of this infection on the degree of intrauterine inflammation and concentrations of progesterone in serum.

Methods

The oestrous cycles of 36 healthy, non-pregnant, postpartum dairy cows were synchronised. They were then treated by intrauterine infusion of 0.5 g cephapirin before being blocked by age and randomly assigned to treatment with intrauterine infusion of saline (n = 18), 10⁷ (n = 9) or 10⁹ (n = 9) cfu of T. pyogenes, approximately 4 days after the expected time of ovulation (Day 0). Prior to intrauterine infusion on Day 0 and again on Days 3, 7, 10, and 15, cytobrush samples were collected from the uterus of each cow for microbiology and assessment of the percentage of polymorphonuclear neutrophils (PMN%). Blood samples were collected on the same days for measurement of concentrations of progesterone in serum, and uterine lumen diameter was assessed daily using transrectal ultrasonography.

Results

Trueperella pyogenes was isolated from 5/18 (28%), 7/9 (78%) and 8/9 (89%) cows infused with saline, 10⁷ or 10⁹ cfu of T. pyogenes, respectively (p < 0.001). Mean PMN% in the control cows did not change over time (p > 0.05), whereas it was higher on Days 7 and 10 than Day 0 in the 10⁷ cfu group, and higher on Days 3 and 10 than Day 0 in the 10⁹ cfu group (p < 0.05). The percentage of observations with uterine lumen diameters >2 mm was higher in cows infused with 10⁷ (29.3 (95% CI = 14.5–44.2)%) or 10⁹ cfu (19.2 (95% CI = 7.0–31.5)%) than in control cows (3.1 (95% CI = 0.1–6.0)%) (p < 0.001). Mean concentrations of progesterone in serum were higher in cows infused with 10⁷ cfu (2.01 (SE 0.19) ng/mL) than cows infused with 10⁹ cfu (1.01 (SE 0.27) ng/mL), with the control group intermediate (1.41 (SE 0.19) ng/mL) (p = 0.03).

Conclusions

Infusion of 10⁷ or 10⁹ cfu of T. pyogenes resulted in the establishment of intrauterine infection in 83% of cows. Infection resulted in increased uterine lumen diameter, and an inflammatory response, i.e. elevated PMN%.

Clinical relevance

This intrauterine infection model may be useful for future research on, for example, the pathogenesis of intrauterine infection in postpartum dairy cows.

KEYWORDS:

• Uterine infection
• postpartum
• dairy cow
• inflammation
• progesterone

Acknowledgements

Microbiology for this study was undertaken by Niki Sowerby of Cognosco, Anexa. Histopathology was undertaken by Dr Alan Julian, IDEXX Laboratories (NZ) Ltd. Animal procedures including bleeding, transrectal ultrasonography, uterine infusion, and uterine sampling were undertaken by Elizabeth Blythe, Stu Morgan, Olivia Jordan, Cassandra Ryder, and Marty Berg. This study was funded by the Dairy Farmers of New Zealand through DairyNZ and by the Ministry for Business, Innovation and Employment.