In vivo metabolism of α-methyltryptamine in rats: Identification of urinary metabolites

Abstract

1. The in vivo metabolism of α-methyltryptamine (AMT), a psychoactive tryptamine analogue, was studied in rats.

2. Male Wistar rats were administered 10 mg kg⁻¹ AMT orally and 24-h urine fractions were collected. After enzymatic hydrolysis of the urine sample, the metabolites were extracted by liquid–liquid extraction and analysed by gas chromatography/mass spectrometry (GC/MS).

3. 2-Oxo-AMT, 6-hydroxy-AMT, 7-hydroxy-AMT and 1′-hydroxy-AMT were detected as metabolites of AMT.

Keywords::

• α-Methyltryptamine (AMT)
• metabolism
• rat
• urine
• gas chromatography/mass spectrometry (GC/MS)