Abstract
1. Human blood was incubated in vitro with a 1:1 mixture of [35 S, 12C4] - and [13 C4] sulphur mustard. Alkylated globin, containing the 2-hydroxyethylthioethyl (HETE) moiety, was isolated from the blood incubate following lysis of the erythrocytes and acidification with HCl in isopropanol. 2. The alkylated globin was hydrolysed with Pronase E to give a digest containing alkylated amino acids and alkylated dipeptides. A number of these were partially purified by hplc and identified by gc-ms and lc-ms. 3. The alkylated globin was hydrolysed with trypsin to give a digest containing alkylatedpeptides. Ten of these were partially purified by hplc, tentatively identified by lcelectrospray mass spectrometry, andthe sequences and sites of alkylation determined using lc-electrospray tandem mass spectrometry. 4. (2-Hydroxyethylthioethyl)glutathione was also shown to be present in the pronase and trypsin digests of alkylated globin. 5. N-terminal valine, on both the alpha and beta chains, andhistidine residues were identified as the key sites of interaction for targeting as biological markers of sulphur mustard poisoning.