Morphological and Functional Expression of Fibroblast on Poly(lactide-co-glycolide)/β-Tricalcium Phosphate/Nature Bone

Abstract

The in-vitro biocompatibility of degradable and bioactive composites consisting of poly(lactide-co-glycolide) (PLGA), β-tricalcium phosphate (β-TCP), and nature bone (NB) was investigated by culturing fibroblasts on the PLGA/β-TCP/NB substrates, and the cell morphology, proliferation, alkaline phosphatase (ALP) activity, and von Kossa mineralization were evaluated. The results showed that the incorporation of β-TCP and NB benefited fibroblasts attachment and the fibroblasts cultured on the PLGA/β-TCP/NB composite substrates spread better as compared to those on the pure PLGA after culturing for different times. MTT assay showed that the fibroblasts cultured on the PLGA/β-TCP/NB extraction revealed a higher proliferation rate than those on the pure PLGA extraction. ALP and von Kossa assay indicated that β-TCP and NB could promote biomineralization in vitro. All of the results showed that the addition of β-TCP and NB into PLGA could stimulate fibroblasts to proliferate and differentiate.

Keywords:

• β-tricalcium phosphate
• biocompatibility
• composite materials
• nature bone
• poly(lactide-co-glycolide)

Acknowledgments

This work was financially supported by the Planning Science and Technology Project of Hunan Province, P. R. China (No. 2010SK3060) and the Scientific Research Fund of Hunan Provincial Education Department (No. 11B045).