Abstract
In this work two phenol biosensors, one based on polyaniline nanofibers (PNFs) and the other based on the newly created and introduced linear-dendritic matrix of poly(citric acid)-block-poly(ethylene glycol) copolymers (PCA-PEG-PCA), were chemically modified with horseradish peroxidase (HRP) enzyme. These phenol biosensors showed an oxidation peak at 0.55 V. The amperometric response for biosensors based on PNFs showed a linear response range from 2.5 × 10−6 to 2.5 × 10−5 mol/L, with a detection limit of 2.5 µM phenol. Also, the amperometric response for a biosensor based on PCA-PEG-PCA showed a linear response range from 2.5 × 10−6 to 4 × 10−5 mol/L, with a detection limit of 1.5 µM phenol.
Acknowledgement
The authors would like to thank the “Iranian Nanotechnology Initiative Council” for their financial support of this work.