In Situ Forming Hydrogel Based on Chondroitin Sulfate–Hydroxyapatite for Bone Tissue Engineering

Abstract

In this research, tissue engineered hydrogels consist of chondroitin sulfate and hydroxyapatite capable of light curing and cell encapsulation were prepared. Chemical and physical characteristics were analyzed by FTIR spectroscopy, compressive strength and water uptake measurements. Observations with fluorescence microscopy as well as MTT viability assay revealed no toxicity for encapsulated cells. Moreover, alkaline phosphatase activity, calcium deposition, and gene expression analysis were determined to prove the osteogenic potential of the prepared hydrogel. In conclusion, this in situ forming hydrogel can be utilized as filler for bone deficiencies as well as carrier for cell encapsulation and osteogenic differentiation.

GRAPHICAL ABSTRACT

Keywords:

• Bone tissue engineering
• differentiation of mesenchymal stem cell
• in situ forming hydrogel
• light cure
• methacrylated chondroitin sulfate