Preparation of temperature-sensitive P (CS-g-TMSPM-g-NIPAAm) copolymer using a chemical cross-linking method

ABSTRACT

A novel thermosensitive P (CS-g-TMSPM-g-NIPAAm) copolymer film was synthesized through grafting thermosensitive monomer N-isopropyl acrylamide (NIPAAm) onto the beforehand modified chitosan (CS) by cross-linking agent 3-trimethoxysilypropyl methacrylate (TMSPM). Fourier transform infrared spectroscopy confirmed the successful polymerization of NIPAAm with CS-g-TMSPM, and its lower critical solution temperature was measured at 34°C. Although osteoblasts and adipose-derived stem cells showed excellent growth state on the film in vitro, only osteoblasts demonstrated good adhesion and stretch probably because of the necessary specific cell–film interplay. Hence, for specific cell cultures and nonenzymatic harvesting in vitro, the prepared thermosensitive film could be a promising approach.

GRAPHICAL ABSTRACT

KEYWORDS:

• Cell adhesion
• chemical cross-linking
• copolymer
• NIPAAm
• temperature-sensitive

Acknowledgments

ADSCs transfected with BMP-2 (P4) were provided as a gift by Dr. Xinjian Qu at our laboratory.