Enhanced chondrogenic differentiation of human bone marrow mesenchymal stem cells on PCL/PLGA electrospun with different alignments and compositions

ABSTRACT

The simultaneous effect of electrospun scaffold alignment and polymer composition on chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMMSC) is investigated. Aligned and randomly oriented polycaprolactone/poly(lactic-co-glycolic acid) (PLGA) hybrid electrospun scaffolds with two different ratios are fabricated by electrospinning. It is found that aligned nanofibrous scaffolds support higher chondrogenic differentiation of hBMMSCs compared to random ones. The aligned scaffolds show a higher expression level of chondrogenic markers such as type II collagen and aggrecan. It is concluded that the aligned nanofibrous scaffold with higher PLGA ratio could significantly enhance hBMMSC proliferation and differentiation to chondrocytes.

GRAPHICAL ABSTRACT

KEYWORDS:

• Cartilage tissue engineering
• chondrocyte
• human bone marrow mesenchymal stem cell
• nanofibrous scaffolds

Acknowledgment

The authors gratefully acknowledge the Stem Cell Technology Research Center and National Institute of Genetic Engineering & Biotechnology for their financial support of this work.

Additional information

Funding

This work was supported by the National Institute of Genetic Engineering & Biotechnology and Stem Cell Technology Research Center.