Soya protein as possible potential nanocarriers for in-vitro oral delivery of insulin in simulated gastric fluids (SGFs)

ABSTRACT

The present study aimed to prepare soya protein nanoparticles and employ them as nanocarriers for delivery of insulin. The nanoparticles were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, scanning electron microcopy, zeta potential, and dynamic light scattering measurements. The particle size analysis revealed that the size of the nanoparticles lays in the range of 40–200 nm with surface charge of −25.3 mV. The insulin-loaded nanoparticles were investigated in simulated gastric and simulated intestinal fluids and effect of percent drug loading chemical composition of nanoparticles, pH, temperature of the release media, and simulated physiologic fluids was studied on the release of insulin.

GRAPHICAL ABSTRACT

KEYWORDS:

• Biopolymers
• insulin
• nanoparticles
• soya protein