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International Journal of Polymeric Materials and Polymeric Biomaterials Volume 68, 2019 - Issue 10
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Articles

PEG-based hydrogel-coated test strip for on-site urea determination

Burcin Alev-TuzunerDepartment of Basic Medical Sciences, Biochemistry, Faculty of Dentistry, Marmara University, Maltepe, Turkey, Istanbul; ORCID Iconhttp://orcid.org/0000-0001-5122-4977View further author information
ORCID Icon,
Asli Beyler-CigilDepartment of Chemistry, Faculty of Arts and Sciences, Marmara University, Goztepe, TurkeyIstanbul; ;Department of Chemistry, Faculty of Arts and Sciences, Amasya University, Amasya, TurkeyORCID Iconhttp://orcid.org/0000-0003-4353-4224View further author information
ORCID Icon,
Memet Vezir KahramanDepartment of Chemistry, Faculty of Arts and Sciences, Marmara University, Goztepe, TurkeyIstanbul; ORCID Iconhttp://orcid.org/0000-0003-1043-6476View further author information
ORCID Icon &
Aysen YaratDepartment of Basic Medical Sciences, Biochemistry, Faculty of Dentistry, Marmara University, Maltepe, Turkey, Istanbul; Correspondence[email protected]
ORCID Iconhttp://orcid.org/0000-0002-8258-6118View further author information
ORCID Icon
Pages 597-606 | Received 23 Feb 2018, Accepted 26 May 2018, Published online: 14 Nov 2018
 
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Abstract

The urea test strip was prepared using polyethyleneglycol based hydrogel, urease and commercial pH-indicator paper. The swelling behavior of hydrogel indicated the ability of the urea to penetrate into the matrix and allowing the test strip to respond in a short time. The color formed on the strip was distinguished by naked eyes in one minute at room temperature. The linear range for urea was 20–200 mg/dL. The assays have good reproducibility. Relative standard deviation values were found within the range of 3.37–12.95% and 1.68–9.54% for intra- and inter-day assays, respectively.

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Additional information

Funding

This work was financially supported by the Marmara University Scientific Research Projects Commission [grant number SAG-B-141014-0344, 2014].

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