Preparation of antibacterial electrospun poly(D, L-lactide-co-glycolide)/gelatin blend membranes containing Hypericum capitatum var. capitatum

Abstract

In this study, we fabricated poly(D, L-lactide-co-glycolide)/gelatin (PLGA/gelatin) membranes containing different amounts of Hypericum capitatum var. capitatum (HCC) extract (1, 5, 7.5, 10 wt%) by electrospinning technique. We investigated chemical, morphological, physical, and mechanical properties as well as in vitro degradation behavior of the electrospun membranes. We also evaluated the antibacterial activity of the electrospun membranes against Escherichia coli and Staphylococcus aureus. Viability, adhesion, and attachment of human fibroblast cells on the electrospun membranes on pre-set days were evaluated by the colorimetric CellTiter 96^® AQueous One Solution Cell Proliferation Assay (MTS assay), scanning electron microscopy (SEM), and 4’,6-Diamidino-2-Phenylindole (DAPI) staining.

Graphical Abstract

Keywords:

• Electrospinning
• Hypericum capitatum var. capitatum
• wound dressing

Acknowledgments

The authors would like to thank Hacettepe University Advanced Technologies Research and Application Center (HUNİTEK), Ankara, for their help in the gas chromatographic and mass spectrometric measurements.

Disclosure statement

No potential conflict of interest was reported by the author(s).