Enhanced proliferation and osteogenic differentiation of mesenchymal stem cells by diopside coated Poly-L-lactic Acid-Based nanofibrous scaffolds

Abstract

In this study, diopside coated Poly-L-lactic acid-based (PLLA/Diopside) and Poly-L-lactic acid along with pluronic acid (PLLA-P123/Diopside) scaffolds were fabricated by electrospinning to improve the drawbacks with pure polymer and pure bioceramic scaffolds in bone tissue engineering application. The surface morphology, hydrophilicity, and mechanical strength were evaluated. The cell proliferation, differentiation, and expression of osteo related genes were evaluated by measuring the basic osteogenic markers and real time polymerase chain reaction, respectively. Diopside coated Poly-L-lactic acid-based nanofibrous scaffolds have improved surface and biological properties making them a good promising candidate for proliferation and osteogenic differentiation in bone repair and regeneration compared to nanofibrous scaffolds without diopside. Especially, post plasma treated diopside coated PLLA demonstrated better osteogenesis and expression of osteo related genes than the other groups. Although, diopside coated PLLA-P123 composite in comparison with PLLA-Plasma/diopside scaffolds were showed less osteogenesis and expression of osteo related genes, but the results were comparable. Therefore, diopside coated (PLLA-plasma and PLLA/P123 composite) scaffolds could be ideal cost effective grafts in bone repair and replacement therapy.

Graphical Abstract

Keywords:

• Bone tissue engineering
• composite scaffold
• diopside
• electrospinning
• osteogenesis
• poly-L-lactic acid
• pluronic P123

Acknowledgment

This study was part of the fulfillment of the Ph.D. thesis of Gebremariam Birhanu supported by the International Campus, Tehran University of Medical Sciences (TUMS-IC).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the Tehran University of Medical Sciences, International Campus (TUMS-IC), Tehran, Iran.