Gentamicin and fluconazole loaded electrospun polymethylmethacrylate (PMMA) fibers as a novel platform for the treatment of corneal keratitis

Abstract

In this study, 10 mg fluconazole (10 FCZ) and 10 mg gentamicin (10 GEN) loaded with 40% polymethylmethacrylate (40% PMMA) fibers were fabricated by electrospinning and the performance of the formulations (40% PMMA, 40% PMMA/10 FCZ, 40% PMMA/10 GEN, and 40% PMMA/10 FCZ/10 GEN) was tested. 10 mg Gentamicin-loaded 40% PMMA fiber mat showed a more significant zone of inhibition against the S. aureus compared to the zone of 40% PMMA/10 GEN/10 FCZ fiber. The biocompatibility test using a human adipose-derived mesenchymal stem cell (MSCs) as a cell model proved that the amount of loaded and released GEN has no toxic effects on the MSCs.

Graphical Abstract

Keywords:

• Corneal keratitis
• electrospinning
• fiber

Acknowledgments

The authors thank Ecem Dogan, Dr. Semra Unal, Dr. Mehmet Mucahit Guncu, Dr. Burak Aksu, and Dr. Ali Sahin for antimicrobial and biocompatibility tests.

Data availability statement

The raw data will be made available upon request.