Preparation of SF/SF-nHA double-layer scaffolds for periodental tissue regeneration

Abstract

Silk fibroin (SF) film/porous SF-nanohydroxyapatite (nHA) double-layer composite scaffolds were fabricated by a combination of electrospinning, casting and freeze-drying. The results showed that the tensile strength and elongation of the SF film layer were significantly improved through the bridging effect of SF/(ε-caprolactone) fibers in the SF matrix. The pore size in SF-nHA porous scaffold layer was approximately 213 μm, and nHA enhanced the compressive strength of the scaffold. CCK-8 assay showed that the SF/SF-nHA scaffold not only had no obvious cytotoxicity but also significantly promoted cell proliferation. The scaffold has the potential to be used for periodontal tissue regeneration.

Graphical Abstract

Keywords:

• Hydroxyapatite
• scaffolds
• silk fibroin
• periodontal tissue

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

We are grateful for the financial support of the National Key Research and Development Program of China [Project No. 2017YFC1103602].