Synthesis, characterization, photocleavage, antimicrobial activity and DNA binding of [Co(bpy)₂MHPIP]³⁺, [Co(dmb)₂MHPIP]³⁺, and [Co(phen)₂MHPIP]³⁺ complexes

Abstract

4-Methyl-2-(2-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthroline) (MHPIP) and its complexes [Co(bpy)₂MHPIP]³⁺ (1) (bpy = 2,2′-bipyridine), [Co(dmb)₂MHPIP]³⁺ (2) (dmb = 4,4′-dimethyl-2,2′-bipyridine), and [Co(phen)₂MHPIP]³⁺ (3) (phen = 1,10-phenanthroline) have been synthesized and characterized by UV/VIS, IR, EA, ¹H, ¹³C-NMR, and mass spectra. The binding of the three complexes with calf-thymus-DNA (CT-DNA) has been investigated by absorption and emission spectroscopy, DNA-melting techniques, viscosity measurements, and DNA cleavage assay. The spectroscopic data and viscosity results indicate that these complexes bind to CT-DNA via an intercalative mode. The complexes also promote photocleavage of plasmid pBR322 DNA and were screened for antimicrobial activity.

Keywords:

• Co(III) complexes
• Polypyridyl ligand
• DNA-binding
• Photocleavage
• Biological activity

Acknowledgement

The financial support from UGC (New Delhi) is gratefully acknowledged.