Genotoxicity of Casiopeina III-Ea in mouse bone marrow cells

Abstract

Casiopeina III-Ea® (Cas III-Ea®) is a chelated copper complex with antineoplastic activity that is capable of reducing tumor size and inducing antiproliferative and apoptotic effects. However, little is known about its in vivo genotoxic effects. Therefore, this study evaluated two cytogenetic and two proliferative parameters 24 h after the administration of Casiopeina III-Ea® to male CD-1 mice. Three doses of Cas III-Ea® were administered by intraperitoneal injections of 1.69, 3.39 and 6.76 mg/kg (corresponding to 1/8, 1/4 and 1/2 of LD₅₀, respectively). A reduction in the mitotic index (MI) and an increased numbers of cells with structural chromosomal aberrations (SCA) were detected. Additionally, a low but significant increase in the frequency of sister chromatid exchange (SCE) was observed at the highest dose. Changes in the DNA replication index (RI) were not observed. These results indicate that Casiopeina III-Ea® shows cytotoxic and clastogenic activity in bone marrow cells from treated mice.

Keywords:

• In vivo cytogenetic
• structural chromosome aberration
• sister chromatid exchange
• mitotic index
• replicative index
• cytotoxicity

Acknowledgements

We thank to Dra. Ma. Elena Bravo-Gómez for the synthesis of Cas III-Ea®, and MVZ. Adriana Altamirano Bautista and MVZ. Roman Hernández Meza for its technical support in the care and handling of animals.

Declaration of interest

The author’s declared no potential conflicts of interest with respect to the research, authorship and/or publication of this article. This work was supported by CONACYT-REDES 87806.