Butea monosperma (Lam.) Taub. Bark fractions protect against free radicals and induce apoptosis in MCF-7 breast cancer cells via cell-cycle arrest and ROS-mediated pathway

Abstract

Butea monosperma (Lam.) Taub. is an ethnomedicinal tree of remedial value in the treatment of diabetes, bone fractures, and liver and neurological disorders. However, the information available on DNA-protective and anti-proliferative potential of bark of this tree is scarce. In the present study, the extract/fractions obtained from bark of B. monosperma were evaluated for antioxidant, DNA-protective, and anti-proliferative activities, along with their phytochemical profiling for identifying major constituents present in them. Different extract/fractions, namely, Bmth (methanol), Bhex (hexane), Bchl (chloroform), and Beac (ethyl acetate), were prepared and evaluated for antioxidant activity using in vitro assays. Extract/fractions were also evaluated for anti-proliferative and apoptotic activity in human breast carcinoma cell line MCF-7, using in vitro assays, namely, MTT, clonogenic, and neutral comet assay, along with confocal microscopy and flow cytometry. Among all extract/fractions, a pronounced antioxidant activity was exhibited by Bchl and Beac fractions, in DPPH· (EC₅₀ 213.2 and 161.5 µg/mL, respectively), ABTS⁺· (EC₅₀ 139.3 and 44.1 µg/mL, respectively), and reducing power assay (EC₅₀ 86.7 and 84.5 µg/mL, respectively). Both fractions protected plasmid DNA against hydroxyl radical induced damage in plasmid nicking assay. Bchl and Beac were also observed to inhibit the growth of MCF-7 cells (GI₅₀ 203.7 and 246.5 µg/mL, respectively). Both fractions induced apoptosis in MCF-7 cells, by arresting the cell cycle in G1 and sub-G1 phase, respectively, enhancing ROS levels, decreasing mitochondrial membrane potential, and inducing double-strand DNA breaks. HPLC analysis revealed high kaempferol content in Bchl, and catechin, epicatechin, and gallic acid in Beac.

Keywords:

• Butea monosperma
• antioxidant
• MCF-7
• ROS
• cell cycle
• apoptosis

Acknowledgments

This work was supported by University with Potential for Excellence (UPE) program under University Grants Commission (UGC), New Delhi (India). The authors thank Dr. Amarjeet Singh Soodan, Department of botanical and Environmental Sciences, GNDU for plant authentication and Professor In charge, Emerging Life Science Centre, GNDU to provide facilities for HPLC, Confocal microscopy, SEM and flow cytometry.

Disclosure statement

No potential conflict of interest was reported by the author(s).

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