Nephroprotective effect of persimmon leaves (Diospyros kaki L.f.) against CCl₄-induced renal toxicity in Swiss Albino rats

Abstract

Diospyros kaki L.f. fruit and leaves are traditionally used for the treatment of hypertension, angina, internal hemorrhage, antithrombotic and anti-inflammatory effects.In the current study, the protective effects of ethyl acetate (Per-1), n-butanol (Per-2), and aqueous (Per-3) fractions of Diospyros kaki leaves against carbon tetrachloride (CCl₄) induced nephrotoxicity in Swiss albino rats were tested. Animal were divided into nine groups; each group consists of six animals. The groups were : group I was untreated and kept as control, group II was treated with CCl₄ only, group III (silymarin with CCl₄); group IV (Per-1 100 mg/kg with CCl₄);group V (Per-1 200 mg/kg with CCl₄); group VI (Per-2 100 mg/kg with CCl₄); group VII (Per-2 200 mg/kg with CCl₄); group VIII (Per-3 100 mg/kg with CCl₄); and group IX (Per-3 200 mg/kg with CCl₄). Silymarin was used as standard drug. All tested fractions were found active (except Per-1 at low dose of 100 mg/kg) with significant value (p < 0.001) compared to CCl₄ only group. Serum creatinine, malondialdehyde (MDA), and uric acid were significantly (p < 0.001) lowered in group VII–IX as compared to CCl₄ only group. Similarly, total protein (TP) and non-protein sulfhydryls(NP-SH) level in kidney tissues were significantly (p < 0.001) elevated in the same groups compared to CCl₄ only group. Further to check the cardio-protective potential, biochemical parameters such as LDH, creatine kinase, TP, MDA, and NP-SH levels in myocardial tissues were also estimated.These findings confirmed that the n-butanol and aqueous fractions are active and recommended for further bioactive phytoconstituents screening. Repeated column chromatography on silica gel G and sephadex-LH-20 of the active n-butanol fraction, four flavonoids were isolated. Based on the spectroscopic NMR data, compounds were identified as kaempferol (1), quercetin (2), astragalin (3), and rutin (4).

Keywords:

• Diospyros kaki L.f.
• n-butanol fraction
• aqueous fraction
• nephro-protective effect
• animal model

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through research group no. RGP-262.