Methanol fraction of Ficus mucoso (welw) prevents iron-induced oxidative damage and alters mitochondrial dysfunction in Drosophila melanogaster

Abstract

The present study investigated the antioxidant and cyto-/mito-protective roles of Methanol Fraction of Ficus mucoso (MFFM) in iron-induced oxidative damage in Drosophila melanogaster. At first, 10-day survival rates were carried out separately on FeSO₄ and MFFM, respectively, after which ameliorative effects of MFFM were investigated on FeSO₄-induced toxicity for 5 days using biochemical and behavioral markers. Additionally, mitochondria were isolated from treated D. melanogaster to assess mitochondrial Permeability Transition (mPT) pore opening. The results showed that FeSO₄ significantly reduced survival rate, total thiol level and activities of catalase and glutathione-S-transferase in D. melanogaster. In addition, treatment with FeSO₄ caused increased generation of H₂O₂, NO (nitrite/nitrates) and acetylcholinesterase (AChE) activity compared with control (p < 0.05). Conversely, MFFM restored FeSO₄-induced inhibition of glutathione-S-transferase and catalase activities, as well as glutathione and total thiol levels. FeSO₄-induced elevation of AChE activity as well as H₂O₂ and NO (nitrites/nitrates) levels were ameliorated by MFFM with improved climbing activity. Interestingly, MFFM prevented FeSO₄-induced mitochondrial Permeability Transition (mPT) pore opening, and elevated mitochondrial ATPase activity and mitochondrial lipid peroxides generation in D. melanogaster. Taken together, our results demonstrated that iron impaired anti-stress defence capacity, altered behavioral functions, increased generation of mitochondrial malondialdehyde and activated opening of the mPT pore in D. melanogaster. Conversely, methanol fraction of F. mucoso protected against iron-induced cyto-/mito-toxic effects. F. mucoso possibly contain bioactive agents which might be useful in the management of disorders associated with oxidative stress induced by iron and or related metals.

Graphical Abstract

Keywords:

• Iron
• oxidative stress
• mitochondria
• Ficus mucoso
• D. melanogaster

Author contributions

Olubukola T. Oyebode: Conceptualization, Validation, Analysis, Writing original draft; Amos Abolaji – Validation, Review and Editing, Supervision; Hammed Faleke: Investigation, Analysis, Writing original draft; Olufunso O. Olorunsogo: Review and Editing, Supervision.

Disclosure statement

The authors declare no conflict of interest.