Biochemical and histopathological analysis after sub-chronic administration of oxyresveratrol in Wistar rats

Abstract

Oxyresveratrol (OXY) is a naturally occurring phenolic compound; however, there are no toxicity studies reported on its long term use. The aim of our work was to demonstrate the evaluation of acute and sub-chronic toxicity of oxyresveratrol in rats to assess its safety profile. To evaluate the LD₅₀ value, 2000 mg/kg of oxyresveratrol was administered to Wistar rats by oral gavage. For sub-chronic toxicity assessment, 80 Wistar rats were randomly divided into four groups (10 animal/sex/group) and oxyresveratrol administered at a dose of 50, 100, 150 mg/kg/day by oral gavage. Bodyweight, food, and water consumption were monitored every week. At the end of the experiments, biochemical and hematological parameters were analyzed. Gross and microscopic organ analyses were also carried out. LD₅₀ of oxyresveratrol was greater than 2000 mg/kg sub-chronic administration of oxyresveratrol did not influence any mortality. Doses of 50 and 100 mg/kg of oxyresveratrol did not produce any sign of toxicity. However, the 150 mg/kg oxyresveratrol group depicted changes in multiple biochemical and hematological parameters with changes in the pathology of cardiac, hepatic, and renal tissues when compared with control. Therefore, no observed adverse effect level (NOAEL) of oxyresveratrol was observed to be 100 mg/kg per day for both male and female rats.

Graphical Abstract

Highlights

• The very first study of oral chronic toxicological assessment of oxyresveratrol.

• Pathological alterations were noted in liver and kidney in 150 mg/kg B.W. per day (High Dose) groups for 90 days.

• No remarkable effects were recorded in Wistar rats for acute oral exposure (2 g/kg B.W.).

• The NOAEL for chronic toxicity of Oxyresveratrol was 100 mg/kg B.W. per day for rats when administered orally for 90 days.

Keywords:

• Oxyresveratrol
• acute toxicity
• sub-chronic toxicity
• safety evaluation
• Wistar rat

Acknowledgements

The authors acknowledge the Maulana Azad National Fellowship for Minorities given by the University Grants Commission for financial support of this study. The authors are also thankful to Dr. A. K. Tiwari for histopathological interpretation, Jamia Hamdard, New Delhi, is acknowledged for providing necessary space and facilities.

Compliance with ethical standards

All pathogen-free male and female animals used in our study were procured from Central Animal House, Jamia Hamdard, New Delhi. All experimental procedures were approved by the Animal Ethics Committee of Jamia Hamdard (173/GO/Re/S/2000/CPCSEA).

Disclosure statement

The authors declare that there is no conflict of interest.

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.