Subacute oral toxicology and toxicokinetics of pterostilbene, a novel Top1/Tdp1 inhibiting anti-tumor reagent

Abstract

This study evaluated the subacute toxicity and toxicokinetics of a potential anti-cancer drug candidate, pterostilbene, in rats. Animals were orally administered at two repeated doses of 200 and 500 mg/kg for 28 days. No mortality was observed during the 28 days of continuous administration of pterostilbene. Body weight and food consumption in each group increased steadily, while no significant difference was found. Liver weight in the 500 mg/kg female, but not male group increased with mild cytoplasmic vacuoles observed in histopathological study. Toxicokinetics was assessed by measuring plasma concentrations of pterostilbene on the first and 28th day of administration using UPLC–MS/MS. Toxicokinetic parameters showed that AUC_0–t significantly increased in all animals, while the increase in females was greater than males. System exposure of pterostilbene appeared to be linear within the administrated dose range. In conclusion, our findings suggested a minimal subacute toxicity profile of pterostilbene, which could strongly support further development of this compound as a novel anti-cancer agent.

Keywords:

• Pterostilbene
• anti-cancer reagent
• subacute oral toxicology
• toxicokinetics
• preclinical study

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This study was supported by the Fundamental Research Funds for the Central Universities (No. 2632020ZD01), Natural Science Foundation of Jiangsu Province (No. BK20190557), and Natural Science Foundation of China (No. 82003877).