Study on the acute and subchronic oral toxicity of Calculus Bovis Sativus in rats

Abstract

This study aims to assess the acute and subchronic toxicity of Calculus Bovis Sativus (CBS), which is an ideal substitute for natural Calculus Bovis. After conducting a test of acute toxicity with KM mice of both sexes, it was determined that oral CBS had a lethal dosage (LD50) of greater than 9.26 g/kg BW. For ninety days, Wistar rats were fed on CBS orally at dosages of 0, 167, 501, and 1503 mg/kg BW/day, respectively, as part of the subchronic investigation. A comparison of the controls with the 1503 mg/kg and 501 mg/kg dosage groups revealed significant differences in the hematological and serum biochemical parameters, such as RBC, HGB, MONO%, PLT, LYMPH% and GLU, TP, ALB, and Ca²⁺, were observed. However, values of the above parameters fell within our laboratory’s normal range. In terms of body weight, food intake, urinalysis, clinical chemistry, and pathology, no other adverse effects were observed. After 90 days of exposure, the no observed adverse effect level (NOAEL) of CBS in rats was determined to be 1503 mg/kg BW/day.

Keywords:

• Acute toxicity
• subchronic toxicity
• no observed adverse effect level
• rats
• Calculus Bovis Sativus

Authors’ contributions

Ying Xia: Methodology, Investigation, Data analysis, Writing – original draft. Yuan: Investigation and data analysis. Xiaoqiao Tang: Investigation of pathology. Jun Fan: Investigation and data analysis. Bolin Fan: Supervision, Methodology. Min Qu: Methodology.

Disclosure statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Data availability statement

Data associated with the study hasn’t been deposited into a publicly available repository. Data will be made available on request.

Additional information

Funding

This research was funded by Hubei Provincial Public Health Academic Leader Program (No: 2021-73).