High Performance Chromatography of Amino Acids, Peptides and Proteins XXIII · Peptide Mapping by Hydrophilic Ion-paired, Reversed-Phase High Performance Liquid Chromatography for the Characterisation of the Tryptic Digest of Haemoglobin Variants

C. A. Bishop Dept. of Chemistry, Biochemistry and Biophysics, Massey University, Palmerston North, New Zealand

W. S. Hancock Dept. of Chemistry, Biochemistry and Biophysics, Massey University, Palmerston North, New Zealand

S. O. Brennan Clinical Biochemistry Dept. Christchurch Hospital, Christchurch, New Zealand

R. W. Carrell Clinical Biochemistry Dept. Christchurch Hospital, Christchurch, New Zealand

M. T. W. Hearn Immunopathology Research Unit Medical Research Council of New Zealand Medical School, P.O. Box, 913, Dunedin, New Zealand

Abstract

The application of high performance liquid chromatography on reversed-phase columns for the tryptic mapping of haemoglobin variants is reported. The effect of flow rate and gradient shape on resolution and column efficiencies has been examined using acetonitrile-water-orthophosphoric acid elution systems. With these conditions it is possible to recognise sequence changes for variant haemoglobins including HbC, HbS, HbE, HbJ Cambridge and HbD Punjab.

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High Performance Chromatography of Amino Acids, Peptides and Proteins XXIII · Peptide Mapping by Hydrophilic Ion-paired, Reversed-Phase High Performance Liquid Chromatography for the Characterisation of the Tryptic Digest of Haemoglobin Variants

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High Performance Chromatography of Amino Acids, Peptides and Proteins XXIII · Peptide Mapping by Hydrophilic Ion-paired, Reversed-Phase High Performance Liquid Chromatography for the Characterisation of the Tryptic Digest of Haemoglobin Variants

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