Ionisation Effects in the Reversed Phase Liquid Chromatographic Separation of Thyromimetic Iodoamino Acids

Abstract

The influence of ionisation equilibria on the retention behaviour of iodoamino acids and related compounds on micro-particulate octadecylsilica supports has been examined. The chromatographic data for these ionogenic solutes have been discussed in terms of current concepts for reversible solvophobic interactions with the hydrocarbonaceous stationary phase. This treatment permits the conditional effects of the mobile phase composition and pH on solute retention to be assessed and the relationship between the molecular surface area of a solute and its retention to a non polar stationary phase evaluated.