Reversed-Phase High-Performance Liquid Chromatographic Separation of Fentanyl Homologues and Analogues. I. An Optimized Isocratic Chromatographic System Utilizing Absorbance Ratioing

Abstract

An optimized isocratic chromatographic system was developed using overlapping resolution mapping for the reversed-phase separation of 26 fentanyl homologues and analogues. The system consisted of a Partisil 10-ODS-3 column with a quaternary mobile phase consisting of phosphate buffer, methanol, acetonitrile and tetrahydrofuran. All 26 compounds were distinguished when UV detection at 215nm was employed in series with UV detection at 230nm.