A Combined HPLC-VIS Spectrophotometric Method for the Identification of Cosmetic Dyes

J. W. M. Wegener Department of Organic Chemistry, Vrije Universiteit, De Boelelaan 1083, 1081, HV Amsterdam, The Netherlands

H. J.M. Grünbauer Department of Organic Chemistry, Vrije Universiteit, De Boelelaan 1083, 1081, HV Amsterdam, The Netherlands; Physical Pharmacy Group, Department of Pharmacy, University of Amsterdam, Plantage Muidergracht 24, 1018, TV Amsterdam, The Netherlands

R. J. Fordham Commission of the European Communities DG XII, Joint Research Centre, Petten Establishment, 1755, ZG Petten, The Netherlands

W. Karcher Commission of the European Communities DG XII, Joint Research Centre, Petten Establishment, 1755, ZG Petten, The Netherlands

Abstract

Ion-pair reversed phase HPLC was observed to give very good separations of 20 representative cosmetic dyes whilst numerical analysis of VIS spectra provided an efficient additional means of identification when similar retention times for different dyes were encountered. The results strongly suggest that a combination of HPLC and rapid scanning VIS spectrophotometry should be very promising, especially when on-line computing facilities are available.

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A Combined HPLC-VIS Spectrophotometric Method for the Identification of Cosmetic Dyes

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A Combined HPLC-VIS Spectrophotometric Method for the Identification of Cosmetic Dyes

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