![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A simple method was developed for the separation of ethyl anthranilate azopigments derived from conjugated bilirubins prepared from germfree (GF) rat feces. The Δ and α0 azopigments were separated and these two azopigments were also separated into their endo-vinyl- and exo-vinyl isomers, respectively. The reverse-phase, high-performance liquid chromatographic (HPLC) separation was achieved by using a μBondapak C18 column and a mixture of acetonitrile, distilled water and sodium acetate as the mobile phase.