Characterization of Phosphoglycerides by Chemical and Enzymatic Hydrolysis on Thin Layer Plates In Situ

Juan G. Alvarez Departments of Obstetrics and Gynecology and Physiology, University of Pennsylvania, Philadelphia, Pennsylvania, 19104

Sidney S. Levin Departments of Obstetrics and Gynecology and Physiology, University of Pennsylvania, Philadelphia, Pennsylvania, 19104

Scott Kleinbart Departments of Obstetrics and Gynecology and Physiology, University of Pennsylvania, Philadelphia, Pennsylvania, 19104

Bayard T. Storey Departments of Obstetrics and Gynecology and Physiology, University of Pennsylvania, Philadelphia, Pennsylvania, 19104

Joseph C. Touchstone Departments of Obstetrics and Gynecology and Physiology, University of Pennsylvania, Philadelphia, Pennsylvania, 19104

Abstract

The use of chemical and enzymatic reactions in situ on thin layer chromatograms for characterization of phosphoglycerides is described. Acid and alkaline hydrolysis under specific conditions can differentiate acyl, alkyl and alkenyl moieties. Phospholipases A₂, C and D were shown to give complete and specific hydrolysis of phospholipids directly on the chromatograms.

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Characterization of Phosphoglycerides by Chemical and Enzymatic Hydrolysis on Thin Layer Plates In Situ

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Characterization of Phosphoglycerides by Chemical and Enzymatic Hydrolysis on Thin Layer Plates In Situ

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