Simultaneous HPLC Analysis of Catecholamines and Indoleamines in Mouse Brain Tissue Following Acetate Extraction and Treatment with Ascorbate Oxidase

M. Gary Hadfield The Neurochemistry Research Laboratory Section on Neuropathology Department of Pathology, Medical College of Virginia Virginia Commonwealth University, Richmond, Virginia, 23298

Christine Milio The Neurochemistry Research Laboratory Section on Neuropathology Department of Pathology, Medical College of Virginia Virginia Commonwealth University, Richmond, Virginia, 23298

Nedathur Narasimhachari The Psychopharmacology Research Laboratory Department of Psychiatry Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia, 23298

Abstract

A refined HPLC Method for the determination of monoamine levels in six brain regions is presented. Analyses are made for the olfactory tubercles, prefrontal cortex, septum, striatum, amygdala and hypothalamus of adult male ICR mice. This system permits the simultaneous analysis of norepinephrine, dopamine, serotonin and their major metabolites during a single run of approximately twenty-five minutes without prior clean-up of samples.

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Simultaneous HPLC Analysis of Catecholamines and Indoleamines in Mouse Brain Tissue Following Acetate Extraction and Treatment with Ascorbate Oxidase

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Simultaneous HPLC Analysis of Catecholamines and Indoleamines in Mouse Brain Tissue Following Acetate Extraction and Treatment with Ascorbate Oxidase

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