Improved High-Speed Countercurrent Chromatograph with Three Multilayer Coils Connected in Series. III. Evaluation of Semianalytical Column with Various Biological Samples and Inorganic Elements

Y. Ito Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, Maryland, 20892

H. Oka Aichi Prefectural Institute of Public Health, Nagoya, Japan

E. Kitazume Visiting Scientist from Iwate University, Morioka, Japan

M. Bhatnagar Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, Maryland, 20892

Y. W. Lee Research Triangle Institute Research, Triangle Park, North Carolina, 27709, U.S.A.

Abstract

The partition efficiency of the counter-current chromatographic centrifuge was improved by mounting a set of three multilayer coils prepared from a 1.07 mm I.D. and ca 300 m length of PTFE (polytetrafluoroethylene) tube with a total capacity of 270 ml. The high performance of the present apparatus has been successfully demonstrated in separations of various testing samples which include DNP (dinitrophenyl) amino acids, indole auxins, tetracycline derivatives, flavonoids, bacitracin, triterpenoic acids, and lanthanoid chlorides.

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Improved High-Speed Countercurrent Chromatograph with Three Multilayer Coils Connected in Series. III. Evaluation of Semianalytical Column with Various Biological Samples and Inorganic Elements

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Improved High-Speed Countercurrent Chromatograph with Three Multilayer Coils Connected in Series. III. Evaluation of Semianalytical Column with Various Biological Samples and Inorganic Elements

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