Foam Countercurrent Chromatography on Various Test Samples and the Effects of Additives on Foam Affinity

Abstract

Foam countercurrent chromatography has been applied to various samples using sodium dodecyl sulfate (SDS), cetyl pyridinium chloride (CPC), and polyoxyethylene-23-lauryl ether (POELE) as foaming reagents. Overall results clearly indicate that the positively charged samples collected with SDS foam and the negatively charged samples with CPC. None of the applied samples showed affinity to POELE. Addition of hydrochloric acid or sodium hydroxide (each at 0.01N concentration) to the surfactant solution effectively reduced the foam affinity of CPC or SDS, respectively, while NaCl at a high concentration of 0.1M showed similar inhibitory action to both surfactants. Among biological test samples, indole-3-acetic acid and abscisic acid were collected with CPC and bovine insulin with SDS foam.